Journal: Mediators of Inflammation

Article Title: Mechanistic Insights Into GDFMD-Mediated Inhibition of Liver Fibrosis via miRNA-29b-3p Upregulation in Wilson's Disease

doi: 10.1155/mi/2776808

Figure Lengend Snippet: The GDFMD inhibits the transdifferentiation of HSCs into myofibroblasts. (A) With GDFMD treatment, the cell activity were detected by CCK8 assay; (B) The mRNA levels of α-SMA, Col1, and TIMP1were checked by qPCR; and (C) The protein levels of α-SMA, Col1, and TIMP1 were checked by WB; (D) The transdifferentiation ability of HSCs into myofibroblasts was evaluated with anti-α-SMA antibody by IF. ⁣ ∗∗ p < 0.01; ⁣ ∗∗∗ p < 0.001.

Article Snippet: After blocking in 5% nonfat milk for 1 h at RT, the membranes were probed with primary antibody against α-SMA (bs-0189R, bioss), Col1 (bs-10423R, bioss), MMP2 (bsm-51472M, bioss), TIMP1 (bsm-10895M, bioss), ULK1 (4773S, CST), Beclin1 (ab210498, abcam), or GAPDH (TA-08, Zshio), followed by HRP-conjugated Goat anti-rabbit/mouse secondary antibody (ZB-2301/ZB-2305, Zsbio).

Techniques: Activity Assay, CCK-8 Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88

doi: 10.1111/jcmm.15119

Figure Lengend Snippet: Primer sequences for quantitative real‐time reverse transcription polymerase chain reaction

Article Snippet: MMP2 and TIMP1 monoclonal antibody were purchased from Boster Bioss. α‐SMA and Col.I monoclonal antibody were purchased from Boster Bioss.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88

doi: 10.1111/jcmm.15119

Figure Lengend Snippet: TMEM88 alleviated ECM accumulation in TGF‐β1‐stimulated LX‐2 cells. A, The mRNA expression levels of MMP2 and TIMP1 were analysed with RT‐qPCR in activated LX‐2 cells transfected with pEGFP‐C2‐TMEM88 and TMEM88‐siRNA, respectively. The results showed that TMEM88 could decrease the mRNA expression level of TIMP1, whereas increase the mRNA expression level of MMP2. B, The protein expression levels of MMP2 and TIMP1 were measured by Western blotting analysis in activated LX‐2 cells transfected with pEGFP‐C2‐TMEM88 and TMEM88‐siRNA. The results showed that TMEM88 could decrease the protein expression level of TIMP1, whereas increase the protein expression level of MMP2. The results were expressed as the mean ± standard of three different experiments. * P < .05 compared with the normal group, # P < .05 compared with the control group

Article Snippet: MMP2 and TIMP1 monoclonal antibody were purchased from Boster Bioss. α‐SMA and Col.I monoclonal antibody were purchased from Boster Bioss.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88

doi: 10.1111/jcmm.15119

Figure Lengend Snippet: MiR‐708 enhanced ECM accumulation on Wnt/β‐catenin signalling pathway in TGF‐β1‐stimulated LX‐2 cells. A, The mRNA expression levels of MMP2 and TIMP1 were analysed with RT‐qPCR in activated LX‐2 cells transfected with miR‐708 mimics and miR‐708 inhibitor, respectively. The results showed that miR‐708 could increase the mRNA expression level of TIMP1, whereas decrease the mRNA expression level of MMP2. B, The protein expression levels of MMP2 and TIMP1 were measured by Western blotting analysis in activated LX‐2 cells transfected with miR‐708 mimics and miR‐708 inhibitor, respectively. The results were expressed as the mean ± SD of three different experiments. The results showed that miR‐708 could increase the protein expression level of TIMP1, whereas decrease the protein expression level of MMP2. C, The protein expression level of β‐catenin, Wnt3a and Wnt2b was performed in activated LX‐2 cells transfected with pEGFP‐C2‐TMEM88 and TMEM88‐siRNA, respectively. The results were expressed as the mean ± standard of three different experiments. * P < .05 compared with the control group, # P < .05 compared with the control group

Article Snippet: MMP2 and TIMP1 monoclonal antibody were purchased from Boster Bioss. α‐SMA and Col.I monoclonal antibody were purchased from Boster Bioss.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot

Journal: Nature Communications

Article Title: A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates

doi: 10.1038/s41467-019-12072-1

Figure Lengend Snippet: WTA galactosylation promotes association of Ami, ActA and InlA to the surface of Lm. a Surface-associated and secreted Lm proteins extracts were obtained from XYSN, ∆1095, and ∆1095::1095. LLO protein levels were used as sample loading control. The image presented is representative of three independent experiments. b Confocal images of XYSN, Δ1095, Δ1095::1095 in Caco-2 BBe cells at 7 h-post infection. ActA was visualized with an anti-ActA monoclonal antibody and goat Anti-Mouse Alexa Fluor 488-conjugated IgG (green), the actin cytoskeleton was stained by phalloidin (red), and DNA was stained by DAPI (blue) following fixation and permeabilization of the sample. Magnification of all images: ×1000. Scale bars, 10 μm

Article Snippet: Proteins were detected using the following antibodies: mouse monoclonal antibody against LLO (3B6) and ActA (6F5) at a 1:2000 dilution, and rabbit polyclonal anti-InlA serum (CSB-PA758331HA01AAD, CUSABIO) at 1:2000 dilution and anti-Ami serum at a 1:5000 dilution – .

Techniques: Control, Infection, Staining